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. 2016 Sep 15;11(9):e0159385. doi: 10.1371/journal.pone.0159385

Fig 2. Systematic evaluation of influence factors to sequencing-based NIPAT.

Fig 2

(A) In given conditions (1000 effective SNPs and 75X sequencing depth), when the fetal fraction increased from 1% to 10%, the CPI increased dramatically; while once fetal fraction reached to 10%, the calculated CPI increased slightly. (B) In given conditions (1000 effective SNPs, 10% fetal fraction), the initial effective sequencing depth changed from 10X to 75X resulted in a dramatic increase of calculated CPI, whereas the following effective sequencing depth change only brought week increase of calculated CPI, and stay stable when the sequencing depth was over 200X. (C) In special conditions (1% fetal fraction, 1000 effective SNPs), deep sequencing (>125X) was recommend for NIPAT. (D) In special conditions (1% fetal fraction, 75X sequencing depth), a larger number of effective SNPs (>10000) was recommend for NIPAT.