Fig 5. E. coli mutant strains harboring adenine at the orthologue of mitochondrial 16S rRNA position 947 showed impaired growth and protein synthesis.

(A) Strains harboring a guanine (black line) or a thymine (red line) grew faster than strains harboring an adenine (green line). Lines represent an average with standard deviation of 42 repeated experiments. (B) E. coli harboring either a thymine (red line) or a guanine (black line) translated YFP more efficiently than E. coli strains harboring an adenine (green line). Lines represent average with standard deviations of 42 repeated experiments. (C) In vitro green fluorescent protein (GFP) synthesis of the different samples was measured over time by fluorescence excitation. Elevated GFP synthesis is observed in strains with ribosomes harboring a guanine (black line) or a thymine (red line) in comparison to strains harboring an adenine (green line). Lines represent average with standard deviation of three repeated experiments. Exact values are available in S1 Data. All experiments were repeated independently by using a second MAGE strain (one for adenine and one for thymine), which gave the same results.