Fig. 4.

Emission-spectral responses of the spectral wide-field microscope. (a) Standard emission spectra of a precalibrated light source (LS-1-CAL). Open circles: intensity spectrum. Shaded circles: photon spectrum. (b) Emission-spectral responses (obtained by comparing the ratio between the measured and standard emission spectrum of the LS-1-CAL) of our system with different dichroic mirrors: D455, D495 and D520. (c) 13 emission-spectral responses of our system with D455 measured from 12 December to 28 December.