Table 1. Compilation of Symbols Used in the Article.
Unit-area-normalized excitation spectrum of X (D for donor, A for acceptor) | ||
Unit-area-normalized emission spectrum of X (D for donor, A for acceptor) | ||
SX | Unit-area-normalized excitation-emission spectral signature of X (D for donor, A for acceptor, S for donor-acceptor sensitization): SD = S ex D⊗ S em D, SA = S ex A⊗ S em A, SS = S ex D⊗ S em A, ⊗: Outer product |
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SDA | Image-stack: Excitation-emission spectrum of FRET sample | |
KX | Total X (D for donor, A for acceptor) absorption in all excitation wavelengths used: ∫Iex εX(λ)dλ | |
(εX(λ) is the extinction coefficient of X at wavelength λ) | ||
rK | The ratio of total acceptor absorption to total donor absorption in all excitation wavelengths used: KA/KD | |
Cy | Concentration of y (d for free donor, a for free acceptor, da for the paired donor-acceptor) | |
C t X | Concentration of total X (D for donor, A for acceptor) | |
QX | Quantum yield of X (D for donor, A for acceptor) | |
rQ | Quantum yield ratio of acceptor to donor: QA/QD | |
WX | Weight of component X (D for donor, A for acceptor, S for donor-acceptor sensitization) | |
E | FRET efficiency of the paired donor-acceptor | |
Eapp | Apparent FRET efficiency (related to total donor): ECda/C t D | |
RC | Concentration ratio of acceptor to donor: C t A/C t D |