FIGURE 3:

MT network rearrangement in the presence of pushing forces. (A) Left, schematic representation of a MT plus end gliding at the cortex and a MT pivoting around its anchor point in the centrosome. MTs and centrosomal complex are in green, actin cortex in red. Right, snapshots of simulations (400 s) covering all the possibilities when pivoting and gliding are independently allowed or not. (B) Trajectories of centrosomes in 15 simulations in which the centrosome was initially positioned at different distances from the cell center and for each of the pivoting/gliding conditions. The center of each disk is marked with a black point. (C) Left, trajectories of centrosomes in simulations with varied pivoting stiffness when gliding is not allowed. Pivoting stiffness is varied geometrically from 0 to 150 pN/μm from left to right along the arc. Right, centrosome positioning as a function of pivoting stiffness, measured by the distance to the cell center. The dashed line indicates the initial centrosome–center distance. (D) Left, trajectories of the centrosome in simulations with varying gliding stiffness when pivoting is not allowed. Gliding stiffness is varied geometrically from 0 to 15.5 pN/μm from left to right along the arc. Right, centrosome positioning as a function of gliding stiffness, measured by the distance to the cell center. The dotted line indicates the initial centrosome–center distance. In all of the plots, the color of the centrosome trajectories indicates time, from blue (0 s) to red (400 s).