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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Oncogene. 2012 Jun 11;32(17):2131–2139. doi: 10.1038/onc.2012.239

Figure 1.

Figure 1

Regulation of Dicer expression by Sox4. (a) Protein extracts were prepared 72 hours after transfection and analyzed for the expression of Dicer and Sox4 by western blot. β-actin was used as a loading control. (b, c) For quantitative reverse transcription PCR analysis, cells were transfected with siCTR, siSox4, empty vector or POTB7-Sox4 and lysed for total RNA extraction and reverse transcription (72 and 24 hours after transfection for siRNA and plasmid transfected cells, respectively). Expression of Dicer and Sox4 mRNAs was measured by real-time quantitative PCR and normalized with β-actin as loading control. *P < 0.05, ***P < 0.001, Student’s t-test. (d) Flag-Sox4 protein binds to the Dicer promoter sequence in MMRU cells in vivo, demonstrated by ChIP assay. (e) Dicer promoter luciferase reporter activity of control and Sox4 knockdown MMRU cells. Whole-cell extracts were prepared 243h after transfection, and luciferase levels were measured by luminometry. All values are expressed as mean±s.d. **P < 0.01.