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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Mol Cancer Res. 2016 Jun 3;14(9):869–882. doi: 10.1158/1541-7786.MCR-16-0007

Figure 2. FAK expression mediates adherent and anchorage-independent growth.

Figure 2

(A) BCPAP and 8505C cells expressing shRNA targeting FAK or a scrambled control were analyzed via Western blot. Blots are representative of 3 independent experiments (B) Viability of BCPAP and 8505C cells expressing shFAK was normalized to scrambled expressing cells, set to 100%. Results are % viable cells per mL ± SD of 3 experiments performed in triplicate (BCPAP shFAK1: p≤0.01 and shFAK2: p≤0.05; 8505C shFAK1 and shFAK2: p≤0.01). (C) Colonies of BCPAP and 8505C cells expressing shFAK were plated in soft agar, allowed to form colonies for 20 days and normalized to scramble cells, set to 100%. Results shown are percent of colony formation ± SD of 3 independent experiments performed in duplicate (BCPAP shFAK1 and shFAK2: p≤0.0001; 8505C shFAK1: p≤0.0001. shFAK2: p≤0.01).