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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Mol Cancer Res. 2016 Jun 27;14(9):795–804. doi: 10.1158/1541-7786.MCR-16-0156

Figure 5. ACF associated stroma have an altered transcriptome with increase immune infiltration.

Figure 5

(A) Heat-map showing supervised clustering analysis. Sixty-eight genes were differentially expressed between ACF stromal samples and normal controls. Six of 9 samples demonstrate a distinct transcriptional profile from normal controls. (B) GSEA determined that cells isolated from the ACF stroma are enriched for gene sets for secreted factors, changes to the extracellular matrisome and enrichment for the senescent fibroblast phenotype. (C) Several pro-inflammatory genes are significantly upregulated in ACF-stroma and are leukocyte and lymphocyte specific. (D) Immunofluorescence of normal and ACF sections confirm increased abundance of CD3+ cells within ACF stroma. Epithelial crypt area is labelled ‘C’ and separated by dashed lines. (E) Quantification of CD3+ cells from 15 high magnification (40x) fields from five ACF and normal pairs