Fig. 2.

ESI-MS (electrospray ionization mass spectrometry) spectrum of the purified NFAP2 (a), and MS/MS spectrum of its N-terminal IATSPYYACNCPNNCK peptide fragment where the first ten amino acids were identified (b). In this peptide C11 and C15 formed an internal disulfide bridge due to the incomplete carbamidomethylation. Amino acid sequence of NFAP2 (c), where arrow indicates the cleavage site of the predicted signal sequence, the first amino acid of the mature protein is marked by an asterisk. Identified peptide by the MS analysis of enzyme digested NFAP2 is underlined. Connected cysteines indicates the predicted disulfide bridge formations