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. 2016 Sep 15;6:75. doi: 10.1186/s13568-016-0250-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — ECD spectra of NFAP2 recorded at 25 °C (black), 95 °C (red), and again at 25 °C immediately (blue), 72 h (green) and 4 weeks (orange) after heat treatment (a). Thermal unfolding curves of NFAP measured at 200 nm (black, R² = 0.9899, T_m = 76.60 °C), 212 nm (red, R² = 0.9770, T_m = 72.49 °C) and 228 nm (blue, R² = 0.9910, T_m = 74.00 °C) (b). RP-HPLC chromatogram of NFAP2 and its comparison with folded and unfolded PAF (c)