Table 1.
In vitro minimal inhibitory concentration (MIC) values of NFAP2, NFAP and conventional antifungal agents for the investigated fungal isolates
| Fungus | MIC (µg ml−1) | ||||||
|---|---|---|---|---|---|---|---|
| NFAP2 | NFAP | AMB | ITC | FLC | TRB | CSP | |
| Yeasts | |||||||
| Candida albicans ATCC 10231 | 0.781 | >200 | 8 | >64 | >64 | 64 | 16 |
| Candida glabrata CBS 138 | 0.391 | >200 | 8 | >64 | >64 | 64 | 16 |
| Candida guilliermondii CBS 566 | 0.391 | >200 | 4 | 2 | 8 | 16 | 16 |
| Candida krusei CBS 573 | 1.563 | >200 | 8 | 2 | >64 | 64 | 16 |
| Candida lusitaniae CBS 6936 | 0.781 | >200 | 8 | 16 | >64 | 16 | 16 |
| Candida parapsilosis CBS 604 | 0.391 | >200 | 8 | 2 | >64 | 16 | 32 |
| Candida tropicalis CBS 94 | 0.391 | >200 | 8 | >64 | >64 | >64 | 8 |
| Saccharomyces cerevisiae SZMC 0644 | 0.195 | >200 | 4 | 2 | 64 | >64 | 16 |
| Schizosaccharomyces pombe SZMC 0142 | 0.391 | >200 | 4 | 8 | 64 | >64 | 16 |
| Filamentous fungi | |||||||
| Aspergillus nidulans FGSC A4 | >25 | 200 | >64 | 4 | >64 | 8 | >64 |
| Aspergillus niger SZMC 601 | >25 | 50 | 8 | 8 | >64 | 16 | >64 |
| Rhizomucor mieheii CBS 360.92 | >25 | >200 | 2 | 1 | 16 | 8 | 8 |
MIC was determined after 24 h (yeasts and R. miehei CBS 360.92) and 48 h (Aspergillus spp.) of incubation. Abbreviations: AMB amphotericin B, CSP caspofungin, FLC fluconazole, ITC itraconazole, NFAP Neosartorya fischeri antifungal protein, NFAP2 Neosartorya fischeri antifungal protein 2, TRB terbinafine