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. 2016 Sep 16;7:314. doi: 10.3389/fphar.2016.00314

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Copyright © 2016 Giacomazzi, Degan, Calabria, Meneguzzi and Minuz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometric analysis of platelet-derived microparticles shed after platelet stimulation with arachidonic acid (AA; 1.25 mmol/L) and in the presence of different concentrations of ASA (100 and 10 μmol/L). Dot plots show the dual fluorescence analysis of representative PFP stained with Annexin V-fluorescein isothiocyanate (FITC) and anti-CD61-phycoerythrin (PE). The total number of CD61+ MPs was calculated as the sum of CD61+/Annexin and CD61+/Annexin + MPs. Absolute counts of PMPs were determined by using Flow-Count^TM Fluorospheres (Beckman Coulter) and expressed per microliter of PFP. Experiments were performed in duplicate.