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. 2016 Sep 16;7:314. doi: 10.3389/fphar.2016.00314

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Copyright © 2016 Giacomazzi, Degan, Calabria, Meneguzzi and Minuz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of antiplatelet agents on in vitro platelet microparticle generation induced by AA and U46619. (A) In vitro PMPs generation induced by AA (1.25 mmol/L) and U46619 (2 μmol/L) in freshly isolated PRP under low shear stress conditions. (B,C) Effects of acetylsalicylic acid (ASA) at high (100 μmol/L) and low (10 μmol/L) concentration on platelet microvesiculation triggered by AA and U46619. (D,E,F) Effects of SQ-29,548 (20 μmol/L), apyrase (10 U/mL) and PSB-0739 (500 nmol/L) on in vitro PMP shedding induced by U46619. (n = 3–20, ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001).