Table 1.
Characteristics of MAPK kinase kinase (MAPKKKs) in Brachypodium.
| Family | Bd gene name | Gene model | Chr | cDNA | Nucleotide features | Protein features | Subcellular localization prediction | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5′UTR | CDS | 3′UTR | Intron | Length | N-ter | C-ter | Kinase Domain | MW (kD) | PI | ||||||
| MEKK subfamily | BdMAPKKK1 | Bradi1g07650 | 1 | 2883 | 128 | 2280 | 475 | 10 | 759 | 372 | 128 | 259 | 82.71 | 9.32 | Peroxisome |
| BdMAPKKK2 | Bradi1g10970 | 1 | 2305 | 0 | 1941 | 364 | 7 | 646 | 375 | 18 | 253 | 69.76 | 5.19 | Chloroplast | |
| BdMAPKKK3 | Bradi1g41850 | 1 | 1701 | 0 | 1701 | 0 | 0 | 566 | 98 | 216 | 252 | 60.54 | 6.29 | Chloroplast | |
| BdMAPKKK4 | Bradi1g58810 | 1 | 2293 | 0 | 2190 | 103 | 10 | 729 | 352 | 118 | 259 | 79.39 | 9.3 | Chloroplast | |
| BdMAPKKK5 | Bradi1g65500 | 1 | 924 | 0 | 924 | 0 | 0 | 307 | 3 | 48 | 256 | 32.29 | 10.41 | Chloroplast | |
| BdMAPKKK6 | Bradi1g67397 | 1 | 2652 | 153 | 1905 | 594 | 7 | 634 | 354 | 27 | 253 | 68.89 | 6.23 | Nucleus | |
| BdMAPKKK7 | Bradi2g17800 | 2 | 1557 | 0 | 1557 | 0 | 2 | 518 | 10 | 257 | 251 | 54.35 | 4.82 | Chloroplast | |
| BdMAPKKK8 | Bradi2g17820 | 2 | 1505 | 77 | 1428 | 0 | 0 | 475 | 10 | 212 | 253 | 50.31 | 4.52 | Chloroplast | |
| BdMAPKKK9 | Bradi2g17830 | 2 | 1650 | 0 | 1650 | 0 | 0 | 549 | 13 | 281 | 255 | 57.92 | 4.44 | Cytoplasmic | |
| BdMAPKKK10 | Bradi2g17840 | 2 | 1326 | 0 | 1326 | 0 | 0 | 441 | 8 | 182 | 251 | 46.63 | 4.74 | Chloroplast | |
| BdMAPKKK11 | Bradi2g47480 | 2 | 1467 | 0 | 1467 | 0 | 0 | 488 | 9 | 229 | 250 | 52.09 | 4.77 | Cytoplasmic | |
| BdMAPKKK12 | Bradi2g47490 | 2 | 1539 | 93 | 1446 | 0 | 0 | 481 | 9 | 225 | 247 | 51.38 | 4.58 | Chloroplast | |
| BdMAPKKK13 | Bradi2g47500 | 2 | 1448 | 65 | 1383 | 0 | 0 | 460 | 9 | 199 | 252 | 48.52 | 4.91 | Cytoplasmic | |
| BdMAPKKK14 | Bradi2g47510 | 2 | 1607 | 96 | 1455 | 56 | 0 | 484 | 9 | 226 | 249 | 52.34 | 5.15 | Mitochondria | |
| BdMAPKKK15 | Bradi3g10887 | 3 | 1419 | 0 | 1419 | 0 | 0 | 472 | 8 | 210 | 254 | 49.01 | 5.04 | Cytoplasmic | |
| BdMAPKKK16.1 | Bradi3g36080.1 | 3 | 2464 | 0 | 2043 | 421 | 16 | 680 | 122 | 298 | 260 | 74.83 | 6.01 | Nucleus | |
| BdMAPKKK16.2 | Bradi3g36080.2 | 3 | 2658 | 0 | 1980 | 678 | 14 | 659 | 122 | 277 | 260 | 72.19 | 5.61 | Nucleus | |
| BdMAPKKK17 | Bradi3g45790 | 3 | 2110 | 0 | 2067 | 43 | 10 | 688 | 287 | 145 | 256 | 75.15 | 9.08 | Mitochondria | |
| BdMAPKKK18 | Bradi3g51380 | 3 | 3104 | 0 | 2676 | 428 | 10 | 891 | 408 | 227 | 256 | 96.73 | 9.62 | Nucleus | |
| BdMAPKKK19 | Bradi3g57740 | 4 | 2143 | 0 | 1587 | 556 | 7 | 528 | 270 | 6 | 252 | 57.82 | 6.96 | Chloroplast | |
| BdMAPKKK20 | Bradi4g22760 | 4 | 1992 | 0 | 1992 | 0 | 10 | 663 | 252 | 155 | 256 | 71.89 | 9.53 | Nucleus | |
| BdMAPKKK21 | Bradi4g29500 | 4 | 2599 | 0 | 2055 | 544 | 16 | 684 | 104 | 318 | 262 | 74.63 | 6.06 | Nucleus | |
| BdMAPKKK22.1 | Bradi5g10670.1 | 5 | 2348 | 0 | 2091 | 257 | 10 | 696 | 291 | 149 | 256 | 75.56 | 9.19 | Nucleus | |
| BdMAPKKK22.2 | Bradi5g10670.2 | 5 | 2345 | 0 | 2088 | 257 | 10 | 695 | 291 | 148 | 256 | 75.48 | 9.19 | Nucleus | |
| BdMAPKKK23 | Bradi5g18180 | 5 | 2691 | 0 | 2691 | 0 | 10 | 896 | 410 | 229 | 257 | 97.56 | 9.7 | Nucleus | |
| BdMAPKKK24.1 | Bradi5g24870.1 | 5 | 4415 | 0 | 4038 | 377 | 23 | 1345 | 18 | 1073 | 254 | 148.9 | 5.93 | Nucleus | |
| BdMAPKKK24.2 | Bradi5g24870.2 | 5 | 4322 | 0 | 3945 | 377 | 24 | 1314 | 18 | 1042 | 254 | 146 | 5.9 | Cytoplasmic | |
| BdMAPKKK24.3 | Bradi5g24870.3 | 5 | 4169 | 0 | 3792 | 377 | 23 | 1263 | 18 | 991 | 254 | 140.9 | 5.87 | Cytoplasmic | |
| Raf subfamily | BdMAPKKK25.1 | Bradi1g04080.1 | 1 | 1770 | 292 | 1152 | 326 | 5 | 383 | 58 | 64 | 261 | 42.45 | 8.11 | Cytoplasmic |
| BdMAPKKK25.2 | Bradi1g04080.2 | 1 | 1773 | 291 | 867 | 615 | 5 | 287 | 59 | 1 | 227 | 31.93 | 8.91 | Cytoplasmic | |
| BdMAPKKK26 | Bradi1g14000 | 1 | 1993 | 463 | 1131 | 399 | 5 | 376 | 71 | 29 | 276 | 41.43 | 8.52 | Cytoplasmic | |
| BdMAPKKK27 | Bradi1g14010 | 1 | 1131 | 0 | 1131 | 0 | 5 | 376 | 71 | 29 | 276 | 41.45 | 8.18 | Cytoplasmic | |
| BdMAPKKK28 | Bradi1g20390 | 1 | 1329 | 0 | 1329 | 0 | 10 | 442 | 165 | 19 | 258 | 49.11 | 7.15 | Cytoplasmic | |
| BdMAPKKK29 | Bradi1g28110 | 1 | 1593 | 0 | 1593 | 0 | 14 | 530 | 257 | 25 | 248 | 59.05 | 6.31 | Chloroplast | |
| BdMAPKKK30.1 | Bradi1g28950.1 | 1 | 3923 | 0 | 3660 | 263 | 7 | 1219 | 942 | 15 | 262 | 134.2 | 5.48 | Nucleus | |
| BdMAPKKK30.2 | Bradi1g28950.2 | 1 | 3692 | 0 | 3660 | 32 | 7 | 1219 | 942 | 15 | 262 | 134.2 | 5.48 | Nucleus | |
| BdMAPKKK31 | Bradi1g30720 | 1 | 3938 | 0 | 3321 | 617 | 8 | 1106 | 833 | 10 | 263 | 118.7 | 5.53 | Nucleus | |
| BdMAPKKK32 | Bradi1g35350 | 1 | 1229 | 0 | 1215 | 14 | 1 | 404 | 106 | 44 | 254 | 45.24 | 9.26 | Chloroplast | |
| BdMAPKKK33.1 | Bradi1g45040.1 | 1 | 3822 | 0 | 3222 | 600 | 12 | 1073 | 799 | 22 | 252 | 117.6 | 5.51 | Chloroplast | |
| BdMAPKKK33.2 | Bradi1g45040.2 | 1 | 3909 | 0 | 3129 | 780 | 11 | 1042 | 799 | 2 | 241 | 114 | 5.39 | Chloroplast | |
| BdMAPKKK34 | Bradi1g47570 | 1 | 4540 | 0 | 4002 | 538 | 8 | 1333 | 1056 | 14 | 263 | 142.2 | 5.75 | Nucleus | |
| BdMAPKKK35 | Bradi1g74480 | 1 | 3521 | 0 | 3006 | 515 | 12 | 1001 | 719 | 30 | 252 | 110 | 6.23 | Chloroplast | |
| BdMAPKKK36 | Bradi2g00670 | 2 | 2080 | 642 | 1113 | 325 | 2 | 370 | 66 | 50 | 254 | 41.07 | 9.19 | Nucleus | |
| BdMAPKKK37 | Bradi2g06260 | 2 | 2635 | 477 | 1686 | 472 | 2 | 561 | 265 | 40 | 256 | 63.4 | 9.36 | Nucleus | |
| BdMAPKKK38 | Bradi2g15560 | 2 | 1414 | 0 | 1140 | 274 | 5 | 379 | 75 | 30 | 274 | 42.12 | 8.01 | Nucleus | |
| BdMAPKKK39 | Bradi2g19590 | 2 | 2466 | 441 | 1788 | 237 | 2 | 595 | 284 | 55 | 256 | 66.38 | 8.5 | Nucleus | |
| BdMAPKKK40 | Bradi2g44910 | 2 | 1477 | 0 | 1164 | 313 | 5 | 387 | 83 | 30 | 274 | 42.92 | 7.69 | Chloroplast | |
| BdMAPKKK41 | Bradi2g46340 | 2 | 2654 | 0 | 2409 | 245 | 15 | 802 | 546 | 8 | 248 | 89.18 | 5.91 | Nucleus | |
| BdMAPKKK42 | Bradi2g49700 | 2 | 2671 | 513 | 1785 | 373 | 2 | 594 | 283 | 55 | 256 | 66.33 | 8.94 | Nucleus | |
| BdMAPKKK43 | Bradi2g49790 | 2 | 1574 | 0 | 1353 | 221 | 11 | 450 | 146 | 48 | 256 | 50.69 | 6.05 | Cytoskeleton | |
| BdMAPKKK44 | Bradi2g57470 | 2 | 1482 | 0 | 1482 | 0 | 10 | 493 | 212 | 21 | 260 | 55.24 | 9.01 | Chloroplast | |
| BdMAPKKK45 | Bradi3g01850 | 3 | 2010 | 0 | 1803 | 207 | 13 | 600 | 323 | 29 | 248 | 66.84 | 6.9 | Cytoplasmic | |
| BdMAPKKK46 | Bradi3g05520 | 3 | 1821 | 247 | 1272 | 302 | 1 | 423 | 142 | 27 | 254 | 46.99 | 7.32 | Cytoplasmic | |
| BdMAPKKK47.1 | Bradi3g08260.1 | 3 | 3209 | 0 | 2562 | 647 | 11 | 853 | 589 | 12 | 252 | 93.22 | 5.45 | Chloroplast | |
| BdMAPKKK47.2 | Bradi3g08260.2 | 3 | 3316 | 0 | 2481 | 835 | 10 | 826 | 589 | 0 | 237 | 90.17 | 5.4 | Chloroplast | |
| BdMAPKKK48.1 | Bradi3g09170.1 | 3 | 2559 | 0 | 2379 | 180 | 15 | 792 | 536 | 8 | 248 | 88.34 | 6.25 | Nucleus | |
| BdMAPKKK48.2 | Bradi3g09170.2 | 3 | 2508 | 0 | 2352 | 156 | 15 | 783 | 536 | 6 | 240 | 87.35 | 6.74 | Nucleus | |
| BdMAPKKK49 | Bradi3g13050 | 3 | 1527 | 0 | 1527 | 0 | 12 | 508 | 261 | 44 | 203 | 57.59 | 5.12 | Nucleus | |
| BdMAPKKK50 | Bradi3g13060 | 3 | 1937 | 0 | 1665 | 272 | 13 | 554 | 285 | 29 | 240 | 62.57 | 6.43 | Nucleus | |
| BdMAPKKK51 | Bradi3g18150 | 3 | 1870 | 193 | 1254 | 423 | 1 | 417 | 136 | 27 | 254 | 45.99 | 8.41 | Cytoplasmic | |
| BdMAPKKK52.1 | Bradi3g27120.1 | 3 | 3316 | 0 | 2907 | 409 | 12 | 968 | 689 | 27 | 252 | 107.1 | 5.84 | Nucleus | |
| BdMAPKKK52.2 | Bradi3g27120.2 | 3 | 3313 | 0 | 2904 | 409 | 12 | 967 | 689 | 27 | 251 | 107 | 5.84 | Nucleus | |
| BdMAPKKK53 | Bradi3g44707 | 3 | 2565 | 0 | 2292 | 273 | 16 | 763 | 493 | 16 | 254 | 83.15 | 7.95 | Chloroplast | |
| BdMAPKKK54 | Bradi3g45660 | 3 | 2196 | 0 | 2196 | 0 | 12 | 731 | 448 | 31 | 252 | 81.79 | 6.89 | Nucleus | |
| BdMAPKKK55 | Bradi3g47600 | 3 | 1550 | 0 | 1053 | 497 | 5 | 350 | 26 | 64 | 260 | 39.42 | 6.97 | endoplasmic reticulum | |
| BdMAPKKK56 | Bradi3g48360 | 3 | 1708 | 0 | 1452 | 256 | 11 | 483 | 202 | 27 | 254 | 53.93 | 8.32 | Chloroplast | |
| BdMAPKKK57 | Bradi3g59510 | 3 | 3683 | 0 | 3345 | 338 | 12 | 1114 | 840 | 22 | 252 | 122.7 | 5.2 | Nucleus | |
| BdMAPKKK58 | Bradi3g60210 | 3 | 3919 | 0 | 3348 | 571 | 7 | 1115 | 842 | 10 | 263 | 122.7 | 5.48 | Nucleus | |
| BdMAPKKK59 | Bradi4g02367 | 4 | 1277 | 0 | 1149 | 128 | 5 | 382 | 74 | 30 | 278 | 41.88 | 5.36 | Chloroplast | |
| BdMAPKKK60 | Bradi4g02900 | 4 | 3653 | 0 | 3231 | 422 | 7 | 1076 | 796 | 17 | 263 | 118.5 | 5.21 | Nucleus | |
| BdMAPKKK61 | Bradi4g04470 | 4 | 2788 | 0 | 2277 | 511 | 12 | 758 | 478 | 28 | 252 | 83.84 | 7.35 | Nucleus | |
| BdMAPKKK62 | Bradi4g09990 | 4 | 2701 | 0 | 2226 | 475 | 12 | 741 | 463 | 26 | 252 | 82.38 | 7.66 | Nucleus | |
| BdMAPKKK63.1 | Bradi4g36880.1 | 4 | 2227 | 116 | 1779 | 332 | 15 | 592 | 311 | 32 | 249 | 65.66 | 5.7 | Cytoplasmic | |
| BdMAPKKK63.2 | Bradi4g36880.2 | 4 | 2296 | 116 | 1743 | 437 | 14 | 580 | 311 | 20 | 249 | 64.48 | 5.48 | Cytoplasmic | |
| BdMAPKKK63.3 | Bradi4g36880.3 | 4 | 2511 | 116 | 1581 | 814 | 13 | 526 | 311 | 2 | 213 | 58.22 | 5.58 | Cytoplasmic | |
| BdMAPKKK64 | Bradi4g38400 | 4 | 2934 | 0 | 2304 | 630 | 15 | 767 | 496 | 17 | 254 | 83.66 | 6.48 | Chloroplast | |
| BdMAPKKK65.1 | Bradi4g41870.1 | 4 | 2062 | 178 | 1617 | 267 | 14 | 538 | 264 | 26 | 248 | 58.75 | 4.91 | Cytoplasmic | |
| BdMAPKKK65.2 | Bradi4g41870.2 | 4 | 1551 | 60 | 1224 | 267 | 12 | 407 | 133 | 26 | 248 | 45.43 | 4.99 | Nucleus | |
| BdMAPKKK66 | Bradi5g21330 | 5 | 3121 | 0 | 2289 | 832 | 14 | 762 | 496 | 12 | 254 | 83.42 | 6.96 | Chloroplast | |
| BdMAPKKK67 | Bradi5g26917 | 5 | 1689 | 0 | 1689 | 0 | 14 | 562 | 285 | 29 | 248 | 62.76 | 5.55 | Cytoplasmic | |
| ZIK subfamily | BdMAPKKK68 | Bradi1g23320 | 1 | 1204 | 0 | 873 | 331 | 1 | 290 | 20 | 12 | 258 | 32.71 | 5.96 | Cytoplasmic |
| BdMAPKKK69 | Bradi1g23970 | 1 | 2678 | 122 | 2043 | 513 | 6 | 680 | 25 | 399 | 256 | 76.88 | 5.51 | Nucleus | |
| BdMAPKKK70 | Bradi2g39347 | 2 | 2381 | 0 | 1932 | 449 | 8 | 643 | 25 | 355 | 263 | 72.45 | 4.88 | Nucleus | |
| BdMAPKKK71 | Bradi3g51457 | 3 | 2106 | 0 | 1848 | 258 | 6 | 615 | 25 | 333 | 257 | 68.33 | 5.02 | Chloroplast | |
| BdMAPKKK72 | Bradi4g41940 | 4 | 1707 | 136 | 1101 | 470 | 4 | 366 | 2 | 145 | 219 | 41.14 | 6.44 | Cytoplasmic | |
| BdMAPKKK73 | Bradi4g44427 | 4 | 2100 | 0 | 1938 | 162 | 3 | 645 | 32 | 340 | 273 | 70.07 | 5.8 | Nucleus | |
Chr, chromosome; UTR, Untranslated regions; CDS, Coding DNA sequence; N-ter, N terninal; C-ter, C terninal; MW, Molecular weight; IP, Isoelectric ponit.