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. 2016 Sep 16;7:361. doi: 10.3389/fimmu.2016.00361

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Copyright © 2016 Jalapothu, Boieri, Crossland, Shah, Butt, Norden, Dressel, Dickinson and Inngjerdingen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prediction of mRNA targets by Ingenuity Pathway Analysis. (A) A network of possible mRNA targets and affected biological pathways for miR-34b and miR-326 was constructed using QIAGEN’s IPA software. Dashed lines represent indirect interactions, while solid lines indicate direct interactions. (B) mRNA expression analysis by qRT-PCR analysis in skin samples from controls (n = 6) or aGvHD rats (n = 6). Samples were normalized to an endogenous control (B2m) and expression levels reported as log of 2^−ΔΔCt ± SD. Significance was calculated using the Student’s t-test (two-tailed) with Welch’s correction.