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. 2016 Sep 16;6:33644. doi: 10.1038/srep33644

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Purified P103 amelogenin-TGF-β1 complex and CF-hTGF-β1 were incubated with porcine MMP20 or KLK4 for 20 h, respectively. (a,c) SDS-PAGE (15% gel) stained with Simply Blue (CBB) showing the P103 amelogenin-TGF-β1 complex (P103 amelogenin) and with silver showing CF-hTGF-β1 (rhTGF-β1). (b,d) The ALP-inducing activity of HPDL cells exposed to the P103 amelogenin-TGF-β1 complex (P103 amelogenin) and CF-hTGF-β1 (rhTGF-β1) samples treated with pMMP20 or pKLK4 (n = 9 culture wells for each sample). Ori: original sample (i.e., no incubation), MMP20: incubation with pMMP20, KLK4: incubation with pKLK4, Cont: incubation without pMMP20 or pKLK4 and β1: recombinant human TGF-β1 with a carrier (0.3 ng mL⁻¹).