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. 2016 Sep 16;6:33647. doi: 10.1038/srep33647

Figure 1. Identification of proteins related to autophagy pathway.

Figure 1

15 μg of proteins from whole cells lysates were loaded and separated by SDS-PAGE. Protein from rat brain was used as positive control for all proteins tested except for LC3 where the positive control was 293T cells with overexpressed human LC3 (10 μl). Immunoblotting was performed, as described in material and methods, with specific antibodies against: Atg 5, Atg 16, p62, LC3 (which recognizes both LC3-I and LC3-II), AMPKα1/2, m-TOR and Beclin 1 proteins.