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. 2016 Sep 16;6:33647. doi: 10.1038/srep33647

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Copyright © 2016, The Author(s)

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Human spermatozoa were incubated for 2 hours at 37 °C in presence or absence of chloroquine (a) and rapamycin (b). Proteins were extracted and resolved by SDS_PAGE. Immunoblotting was performed with anti-LC3 antibody (described in materials and methods section). Results are expressed as increase of LC3-II/LC3-I ratio respect to control samples (containing only vehicle). Columns with asterisk indicate significant differences (P < 0.05) respect to control. n = 4. CQ: chloroquine; RAP: rapamycin.