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. 2016 Jul 30;291(38):19746–19759. doi: 10.1074/jbc.M116.736876

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Hem25p deficiency impairs the expression of cytochrome bc₁ complex as evaluated by specific antibodies for cytochrome b, cytochrome c₁, Cor1, and Cor2. Mitochondria (30 μg of protein) were extracted from wild-type (lane 1), hem25Δ (lane 2), HEM25-pYES2 (lane 3), and SLC25A38-pYES2 (lane 4) and separated by SDS-PAGE, transferred to nitrocellulose, and immunodetected with antibodies directed against the Cyt b (A), Cyt c₁ (B), Cor1 and Cor2 (C), and Dic1 (D) proteins. Anti-Dic1 was used as the control.