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Subcellular localization of TbKH in BF parasites using a C-terminal HA3 epitope tag. Localization of TbKH::HA3 in isolated flagella (A), subpellicular microtubules (B), and mitotic spindles and subpellicular microtubules (C), prepared as described for Fig. 1 but employing anti-HA mAb for immunostaining. Tub, α-tubulin.
