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Distribution of the ECL4-ECL5 distances in OCCnK (A) and SMDnK (B) simulations. The calculations were performed on three separate parts of the respective 2-μs-long trajectories: 1) the time interval when K+ is stably bound in the Na2 site (INITIAL, red curve); 2) the time interval when K+ becomes destabilized (TRANS, green curve); and 3) after K+ leaves the transporter (FINAL, blue curve).
