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. 2016 Jul 27;291(38):19953–19961. doi: 10.1074/jbc.M116.731612

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Ratios R_i/R_ad as a function of Mg²⁺ concentration. Ratios of contact radius to initial vesicle radius R_i/R_ad of GUVs composed of DOPC/DOPE/cap-biotin-PE/Atto488-DPPE (67:30:2:1) (A) and DOPC/DOPE/cap-biotin-PE/Atto488-DPPE/PtdIns(4,5)P₂ (66.2:30:2:1:0.8) (B) adhered to an avidin-coated glass surface measured at different Mg²⁺ concentrations. For each condition 31–101 GUVs (n_total = 413, A) and 79–117 GUVs (n_total = 467, B) were measured obtained from at least two independent preparations for each condition. After 2 h of incubation the same values were obtained (see in B, after 2 h). Black diamonds represent the mean. ***, p < 0.001; *, p < 0.05; ns, not significant.