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Tubulation after ENTH binding to a GUV with low membrane tension. Time series of three-dimensional reconstructions of SDCLM images of a GUV (DOPC/DOPE/cap-biotin-PE/Atto488-DPPE/PtdIns(4,5)P2, 66.2:30:2:1:0.8) adhering on an avidin-coated glass surface at low membrane tension (c(Mg2+) = 0.5 mm). The GUV starts to tubulate after ENTH addition (cENTH = 1 μm) and forms tethers until all lipid material is dissipated. Tubulation starts at t = 0 min. Scale bars: 10 μm.
