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Production of native and mutated forms of HMA6 and HMA8 in Lactococcus membranes. Total membrane proteins (20 μg) were separated by SDS-PAGE. The different forms of HMA6 and HMA8 were detected using a Strep-tactin HRP conjugate. The arrow indicates HMA6, HMA6-C161A, HMA6-H165A, HMA8, and HMA8-H193A. EV, membrane extract derived from bacteria containing the empty pNZ8148 vector.
