FIGURE 4.

Importance of the TAB3-binding domain to NleE function during infection. A, immunoblot of IκB degradation in HeLa cells infected with derivatives of EPEC E2348/69 for 2 h and left unstimulated or stimulated with TNF for 30 min. B and C, IL-8 production from HeLa cells infected with derivatives of EPEC E2348/69 as indicated for 2 h and left unstimulated or stimulated with TNF for 8 h. Results are the mean ± S.E. (error bars) of three independent experiments carried out in duplicate. *, significantly different from unstimulated HeLa cells or HeLa cells stimulated with TNF and infected with EPEC E2348/69 (p < 0.02, unpaired two-tailed t test). D, [³H]AdoMet (SAM) labeling of full-length TAB3 immunopurified from HEK293T cells and incubated with either purified GST-NleE or GST-NleE^49AAAA52 in the presence of [³H]AdoMet. [³H]AdoMet labeling was determined by ³H autoradiography (auto).