Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 21;291(38):20149–20162. doi: 10.1074/jbc.M116.734079

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Binding of NleE to TAB2 and ZRANB3. A, immunoprecipitation of TAB2-FLAG and detection of EGFP-NleE and EGFP-NleE^49AAAA52 binding in HEK293T cells. Actin is a loading control. B, immunoprecipitation (IP) of ZRANB3-FLAG and detection of EGFP-NleE and EGFP-NleE^49AAAA52 binding in HEK293T cells. Actin is a loading control. C and D, [³H]AdoMet (SAM) labeling of full-length TAB2-FLAG or ZRANB3-FLAG as indicated immunopurified from HEK293T cells and incubated with purified GST-NleE or GST-NleE^49AAAA52 in the presence of [³H]AdoMet. [³H]AdoMet labeling was determined by ³H autoradiography (auto).