FIGURE 7.

S-Palmitoylation of Cys-265 and cAMP production induced by stimulation with a battery of pharmacological agents. A, S-palmitoylation of Cys-265 was enhanced by the endogenous β₂AR ligand epinephrine and the synthetic agonist fenoterol but not by norepinephrine, procaterol, or salbutamol (10 μm, 2 h). n = 6; *, p < 0.05 versus unstimulated control by ANOVA. B, all agents employed in A (10 μm, 5 min) enhanced cAMP production to a similar extent. C, none of a battery of 14 additional agents acting on the β₂AR enhanced the S-palmitoylation of Cys-265, including carvedilol, which signals through β-arrestins (10 μm, 2 h).