FIGURE 2.

Overexpression of pre-miR-223 significantly improved cardiac function recovery during ex vivo I/R. A, diagram of pre-miR-223 TG vector. B–D, overexpression of miR-223-3p/-5p was determined in TG hearts by quantitative RT-PCR and 3% agarose gel electrophoresis. U6 was used as an internal control (*, p < 0.001; n = 3 for each group). E–H, pre-miR-223 TG hearts showed better functional recovery than WT hearts after 45-min global ischemia followed by 1-h reperfusion (n = 8 for WT; n = 7 for pre-miR-223 TG group; *, p < 0.005 versus WT). Error bars represent mean ± S.D. α-MHCp, α-myosin heavy chain promoter. hGHpA, human growth hormone poly A.