FIGURE 8.

Acute overexpression of miR-223-3p/-5p in adult rat cardiomyocytes protected against H₂O₂-induced cell death. A, constructs of Ad.miR-223 and control Ad.β-gal. B, nearly 100% of cardiomyocytes were infected by Ad.miR-223 and Ad.β-gal. C, real time PCR showed overexpression of miR-223-5p and -3p in Ad.miR-223-infected cells. D and E, cardiomyocyte survival and LDH release were measured using MTS incorporation and an LDH analysis kit, respectively. F and G, representative Western blots and quantitative results of the necroptotic signaling cascades (RIP1/RIP3/MLKL) in Ad.miR-223- or Ad.β-gal-infected cardiomyocytes upon H₂O₂ treatment (n = 4; *, p < 0.05 versus Ad.β-gal control). Error bars represent mean ± S.D. Buff, buffer.