FIGURE 1.

Interaction of PexRD54 and ATG8CL proteins in vitro. A, analytical gel filtration traces obtained for PexRD54 (top), ATG8CL (middle), and a 1:1 mixture of the complex (bottom). Insets show SDS-polyacrylamide gels of the fractions collected across the elution peaks. B, gel filtration trace derived from preparative purification of the PexRD54-ATG8CL complex following co-expression in E. coli. Inset, SDS-polyacrylamide gel containing purified complex. C, binding curve derived from SPR single cycle kinetics data for PexRD54 binding to ATG8CL.