Skip to main content
. 2016 Jul 25;291(38):20270–20282. doi: 10.1074/jbc.M116.744995

FIGURE 4.

FIGURE 4.

Crystal structure of ATG8CL bound to the PexRD54(377–381)-peptide and specificity of peptide binding. A, schematic representation of ATG8CL/PexRD54(377–381)-peptide complex highlighting key interactions. ATG8CL is shown in magenta schematic representation with the molecular surface that contacts the PexRD54(377–381)-peptide shown in orange. The PexRD54(377–381)-peptide is shown as sticks with yellow carbon atoms. The electron density omit map of the peptide ligand (Fobs − Fcalc map) is shown in blue mesh and contoured at 2 σ. Electrostatic interactions are indicated with black dashed lines. B, results of the peptide array analyzing the effect of single amino acid substitutions (top) at all positions of 10-mer peptide of PexRD54 (Lys-372–Val-381, side). GST-tagged ATG8CL was visualized using an anti-GST-HRP antibody.