FIGURE 6.

PexRD54 and PexRD54-ATG8CL complex analyzed by small angle x-ray scattering. A, fits of the most probable (lowest NSD) dummy atom models from DAMMIN for PexRD54 (left) and PexRD54/ATG8CL (right). The fit to the experimental data (in black) is shown in wheat and cyan, respectively, with χ² shown as an inset. B, superposition of the crystal structure of PexRD54 with the most probable ab initio envelope of PexRD54 (wheat surface). C, superposition of the CORAL rigid body model of PexRD54/ATG8CL + pentapeptide with the most probable ab initio envelope of the complex (cyan surface). For B and C, two views are shown, face-on (left) and end-on (right). The fits shown in A and the envelopes shown in B and C are from the same run of DAMMIN.