FIGURE 8.

Interaction of PexRD54^Δ218 and PexRD54^Δ298 with ATG8CL in vitro and in planta. The binding affinities of PexRD54^Δ218 (A) and PexRD54^Δ298 (B) to ATG8CL were determined by ITC. Following a heats-of-dilution correction, a single-site binding model was used to fit the data using the MicroCal Origin software (data are shown on the top, with the fit on the bottom). The insets in the top panel depict the PexRD54 truncation used in the experiment, colored as in Fig. 3A. C, validation of PexRD54^Δ218 and PexRD54^Δ298 interaction with ATG8CL in plant cells by co-immunoprecipitation. Red asterisks indicate expected band sizes of the PexRD54 constructs. Degradation is due to autophagy, as seen previously (35).