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. 2016 Sep 16;6:33039. doi: 10.1038/srep33039

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Representative images from sphere formation assays after the depletion of USP16 in Huh7 and PLC/PRF/5 cells are shown (bar, 100 μm). (B) The diameters of spheres of different groups were measured. The data represent the mean ± S.D, **p < 0.01, ***p < 0.001, determined by t-test. (C,D) Huh7 and PLC/PRF/5 cells with or without USP16 down-regulation were treated with 5-fluorouracil (C) and adriamycin (D). Cell viabilities were measured three days after drug treatment, and IC50 values were calculated and were tested using one-way ANOVA. The data represent the mean ± S.D. (E) The relative mRNA expression levels of the indicated stemness-related genes were analysed using real-time PCR in control and USP16-depleted Huh7 cells. Mean ± S.D are shown, *p < 0.05, **p < 0.01, ***p < 0.001, determined by t-test.