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. 2016 Sep 1;7:12569. doi: 10.1038/ncomms12569

Figure 7. A synergistic effect of a paternal mitochondrial fission defect and a maternal mitochondrial fusion defect in delaying PME.

Figure 7

(a,d) Quantification of MTR-stained paternal mitochondrial clusters in different stages of cross-fertilized embryos from the indicated crosses. In all cases, MTR-stained males were mated with unstained hermaphrodites. Data shown are mean±s.e.m. (n=10). (b,e) PCR-based assays to monitor paternal uaDf5 mtDNA in different stages of cross-fertilized embryos or animals from the indicated crosses were performed as in Fig. 2i. Each PCR assay was prepared from eight cross-fertilized embryos or animals (see Methods). Uncropped gel images are shown in Supplementary Fig. 7. (c,f) Quantification of MTR-stained paternal mitochondrial clusters in 16- and 32-cell stage (c) or comma and 4-fold stage (f) cross-fertilized embryos from the indicated crosses. Data shown are mean±s.e.m. (n=15). MTR-stained males were mated with unstained hermaphrodites (a,c,d,f). The significance of difference between different mating experiments was determined by unpaired t test. *P<0.05, **P<0.01. ‘n.s.' indicates no significant difference. In all panels, atg-7(bp422), drp-1(tm1108) and fzo-1(tm1133) alleles were used.