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. 2016 Sep 16;6:32886. doi: 10.1038/srep32886

Figure 3. Generation of quadruple eukaryotic translation initiation factor 2 (eIF2α) kinase knockout mouse embryonic fibroblasts (MEFs) (4KO) by CRISPR/Cas9-mediated genome editing.

Figure 3

(A) Schematic of each targeting locus of the four Eif2ak kinases with the target sequence underscored, PAM in bold type and exons in block boxes. Representative sequencing results showing the indel mutations generated in 4KO cells. (B) Representative immunoblots of protein kinase R (PKR), PKR-like ER kinase (PERK), general control non-depressible 2 (GCN2) and ribophorin (Rb) in the wild-type and 4KO MEFs. (C) RT-qPCR analysis (mean ± SD, n = 4, *p < 0.01) of the expression of heme-regulated inhibitor (Hri) mRNA in the wild-type and 4KO MEFs.