Skip to main content
. 2016 Sep 16;6:32886. doi: 10.1038/srep32886

Figure 5. Quadruple eukaryotic translation initiation factor 2 (eIF2α) kinase knockout mouse embryonic fibroblasts (4KO MEFs) overexpressing the indicated Flag-tagged eIF2α kinases are useful tools for validating the activation of each eIF2α kinase by various stressors.

Figure 5

(A) Representative immunoblots showing ectopically expression of the indicated Flag-tagged eIF2α kinases and endogenous ribophorin (Rb) in the six types of MEFs (wild-type, 4KO and 4KO overexpressing the indicated Flag-tagged eIF2α kinases). (B) Representative immunoblots of protein kinase R (PKR), PKR-like ER kinase (PERK), general control non-depressible 2 (GCN2) and Rb in the six types of MEFs (wild-type, 4KO and 4KO overexpressing the indicated Flag-tagged eIF2α kinases). (C) RT-qPCR analysis (mean ± SD, n = 4, *p < 0.01) of the mRNA expression of heme-regulated inhibitor (Hri) in the six types of MEFs (wild-type, 4KO and 4KO overexpressing the indicated Flag-tagged eIF2α kinases). (D) Representative immunoblots of phosphorylated eIF2α, total eIF2α and Rb 1 h after treatment with the indicated stress stimulus in the six types of MEFs (wild-type, 4KO and 4KO overexpressing the indicated Flag-tagged eIF2α kinases).