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. 2016 Sep 1;7:12649. doi: 10.1038/ncomms12649

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Flow of the experiments. Wing discs from wing-size-extreme Drosophila inbred lines were dissected and collected. SWATH-MS quantified wing disc proteomes for each line/sex, which were analysed to identify/characterize wing-size-associated proteins. (b) Reproducibility of the experiment. Pairwise Spearman's rank correlation coefficients between peptide levels showed higher correlations among biological replicates than among non-replicates. (c) Variation of protein levels; s.d. is plotted in an increasing manner. (d) Relationship between protein variation and protein abundance. Less abundant proteins show larger variations. (e) Cluster analysis of the proteome data matrix. Proteins (1,610 entries) and samples (30 lines × 2 sexes) are hierarchically clustered based on Spearman's correlations.