Figure 3. ORP4L is required for PLCβ3 activation.

(a) Specific [³⁵S]GTPγS exchange in Gα_q/11 in Jurkat T-cells with ORP4L knockdown or overexpression. Cells were stimulated with or without 10 μg ml⁻¹ anti-CD3 for 5 min before analysis. (b) PLCβ3 phosphorylation and PLC activity in Jurkat T-cells with Gα_q/11 knockdown induced with or without 10 μg ml⁻¹ anti-CD3 for 5 min. (c,d) PLCβ3 phosphorylation and PLC activity in Jurkat T-cells (c) and Molt-4 cells (d) with ORP4L knockdown or overexpression. Cells were stimulated with 10 μg ml⁻¹ anti-CD3 for 5 min. (e) IP₃ production in Jurkat T-cells, Molt-4 cells and primary T-ALL cells (n=3) with ORP4L knockdown (left) or overexpression (right). Cells were stimulated with 10 μg ml⁻¹ anti-CD3 for 5 min. The data represent mean±s.d. value from an experiment performed in triplicate, **P<0.01, ***P<0.001, Student's t test.