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. 2016 Sep 2;7:12706. doi: 10.1038/ncomms12706

Figure 1. Nestin+ cells regulate inflammatory cell traffic in atherosclerosis.

Figure 1

(a) Flow cytometry plots showing the cell sorting strategy. (b) QPCR analysis of Icam1, Vcam1 and Mcp1 mRNA in BM CD45− Ter119− CD31+ endothelial cells (BMEC) and BM CD45− Ter119− CD31− stromal cells (BMSC) from ApoE−/− mice fed with chow or HFD for 2 months (n=3–7). (c) CD31 expression in BM GFP+ stromal cells from Nes-gfp;ApoE−/− mice fed with HFD for 2–3 months (n=4). (d) Experimental design of peripheral blood analysis from mice treated with tamoxifen and fed with HFD for 2 months. (e,f) Flow cytometry strategy used to identify inflammatory neutrophils, inflammatory monocytes and non-classical monocytes. (g,h) Number of non-classical monocytes, inflammatory monocytes and inflammatory neutrophils in the peripheral blood of tamoxifen-treated (g) Nes-CreERT2;Mcp1f/f;ApoE−/− mice, (h) Cdh5-CreERT2;Mcp1f/f;ApoE−/− mice and control littermates (n=3–12). Data are means±s.e.m.; n and P values are indicated; *P<0.05, unpaired two-tailed t test.