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. 2016 Sep 16;36(5):e00379. doi: 10.1042/BSR20160118

Figure 2. Additive inductions by IL-1β and HMGB1 of PGE2, TNF-α, IL-6 and IL-8 in disc annulus fibrosus cells.

Figure 2

Disc annulus fibrosus cells were treated with 2 ng/ml IL-1β or (and) with 40 ng/ml HMGB1 for 0, 6, 12, 24 or 48 h, then the supernatant levels of PGE2 (A), TNF-α (B), IL-6 (C) and IL-8 (D) were examined with ELISA methods. Results are presented as mean ± S.E.M. for quartic independent experiments. P values were calculated by parametric ANOVA (Tukey–Kramer post hoc) test. *P<0.05, **P< 0.01, ***P<0.001 or ns: no significance.