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. 2016 Sep 16;36(5):e00379. doi: 10.1042/BSR20160118

Figure 6. Regulation on NF-κB/p65 and IκBα by IL-1β and HMGB1 in disc annulus fibrosus cells.

Figure 6

(A) Disc annulus fibrosus cells were treated with 2 ng/ml IL-1β or (and) with 40 ng/ml HMGB1 for 12 h, then the relative mRNA levels of NF-κB/p65 and IκBα to GAPDH were examined with RT-qPCR method. (B and C) Western blotting assay for (B) and relative levels of (C) NF-κB/p65 and IκBα in the disc annulus fibrosus cells, which were treated with 2 ng/ml IL-1β or (and) with 40 ng/ml HMGB1 for 24 h, with GAPDH as control; Student t test was performed for statistical significance analysis. *P<0.05, **P<0.01 or ns: no significance.