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| G2l wt | G2l D187N | G2l N184K | G2s N184K | |
|---|---|---|---|---|
| Tm (°C) | Tm (°C) | Tm (°C) | Tm (°C) | |
| +Ca | 60.6 | 46.5 | 45.6 | 47.0 |
| −Ca | 41.5 | 46.4 | 33.4 | 33.8 |
| ΔTm | 19 | 0 | 12 | 13 |
Tm values (±0.3 °C) are reported for the G2l construct of the wt protein and mutants D187N and N184K and the short construct of mutant N184K. Measurements were performed in the presence of either 1 mM calcium chloride (+Ca) or 1 mM EDTA (−Ca).
