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. Author manuscript; available in PMC: 2016 Sep 16.
Published in final edited form as: Circ Res. 2014 Sep 16;115(11):911–918. doi: 10.1161/CIRCRESAHA.115.304267

Figure 3. Knockdown of PKCβ prevents TNF-α-mediated VSMC activation.

Figure 3

(A) Validation of PKCβI silencing at the protein level. WT murine VSMCs were treated with control (Ctrl) or PKCβ siRNA followed by treatment with TNF-α and assessment of (B) Nox1 phosphorylation (C,D) CM-H2DCF fluorescence (RFU: relative fluorescent units; n=4–7; scale bar = 10 µm), and (E) migration (n=10–15) as in Figure 2. In (C), nuclei were counter-stained with ToPro3 (blue). *p<0.05 vs. vehicle; #p<0.05 vs. siCtrl TNF-α-treated.