Figure 4.

CM expression results in reduced homeostatic erythroid differentiation in vivo. (A) Schematic of experimental design. Cbfb^+/56M/Mx1-Cre/Rosa26^mT/mG (CM) or control Mx1-Cre/Rosa26^mT/mG (Ctrl) dual fluorescent reporter mice were injected with 7 doses of poly (I:C) to induce the expression of Cre. Contribution of CM-expressing cells (GFP⁺) in HSPC subsets were assessed 6 weeks after induction. (B) Representative FACS plots of GFP⁺ (CM-expressing) and dTomato⁺ (non-CM cells) in various phenotypic HSPC subsets. (C) Frequency (%) of GFP⁺ cells in each phenotypic compartment of CM (n = 7) or Ctrl mice (n = 9). Mean ± SEM is shown. (D) Frequencies of GFP⁺ LSK and myeloid/erythroid progenitor subsets within total CM (n = 6) or Ctrl (n = 6) BM. Mean ± SEM is shown. *P < .05; **P < .01; ***P < .001.