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. 2016 Aug 22;197(7):2838–2853. doi: 10.4049/jimmunol.1501923

FIGURE 1.

FIGURE 1.

C57BL/6 macrophages produce higher levels of IL-10 but lower levels of proinflammatory cytokines compared with BALB/c macrophages in response to bacterial products. C57BL/6 and BALB/c BMDMs were stimulated with B. pseudomallei, LPS, or Pam3CSK4 for the indicated times. IL-10 (A), IL-12p40, IL-12p70 (B), TNF-α (C), and IL-1β (D) protein levels in supernatants were determined by ELISA. Il12a mRNA expression (B) was determined by qRT-PCR and normalized to Hprt1 mRNA expression. Graphs show means ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by two-way ANOVA (Bonferroni multiple comparison test). Bps, B. pseudomallei.