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. 2016 Aug 22;197(7):2838–2853. doi: 10.4049/jimmunol.1501923

FIGURE 5.

FIGURE 5.

Type I IFN promotes B. pseudomallei– and LPS-stimulated IL-10 production transcriptionally and by stabilization of Il10 mRNA in C57BL/6 and BALB/c macrophages. (A and B) C57BL/6, BALB/c, and C57BL/6 Ifnar1−/− BMDMs were stimulated with B. pseudomallei or LPS for the indicated times. (C) C57BL/6 and C57BL/6 Ifnar1−/− BMDMs were stimulated with LPS for 1 h and treated with actinomycin D (ActD). (A–C) Il10 mRNA was harvested and quantified by qRT-PCR, normalized to Hprt1 mRNA levels. (D) C57BL/6 and BALB/c BMDMs were treated with 2 or 20 ng/ml IFN-β for 2 h prior to stimulation with B. pseudomallei or LPS for 24 h. IL-10 production was quantified by ELISA. Graphs show means ± SEM of two to three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 by two-way ANOVA (*C57BL/6 versus BALB/c, +C57BL/6 versus C57BL/6 Ifnar1−/−), Bonferroni multiple comparison test.