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. Author manuscript; available in PMC: 2016 Sep 16.
Published in final edited form as: Radiat Res. 2015 Dec 3;184(6):639–649. doi: 10.1667/RR14178.1

Figure 3.

Figure 3

Characterization of IMs. Lung cells from C3H and C57 mice, collected 3–26 wk following exposure of to 0 Gy or 12.5 Gy whole lung irradiation, were analyzed by flow cytometry. 3A: IMs were identified by first gating on autofluorescent− cells in the CD45+, Ly6G− population and were identified as CD11b+ and CD11c+. IMs were next analyzed for their expression of F4/80, Ly6C and CD206. One representative sample is shown. 3B: Radiation-induced alterations in IM cell number. 3C: Radiation-induced alterations in the proportion of CD206 and F4/80+ IMs. 3D: Radiation-induced alterations in the proportion of Ly6C and F4/80+ IMs. Each bar represents mean ± SEM (n = 3–4 mice/treatment group). aSignificantly different from age-matched controls. bSignificantly different from 3 wk time point (controls only). cSignificantly different from C3H mice.