Figure 1.

Schematic diagram of the eIF(iso)4E locus targeted for editing by CRISPR/Cas9. Primers flanking the target site are shown by half arrows over the 5′ untranslated region (UTR) and open reading frame (ORF) shaded grey and blue, respectively. The scale bar depicts the approximate positions, in base pairs, relative to the translation start site (+1). The enlarged area indicated by the red broken line shows the position and sequence of the sgRNA (single guide RNA), with the protospacer adjacent motif (PAM) underlined. The position of the sgRNA relative to the +1 of the ORF is indicated by an arrow. The Cas9 cleavage site is indicated by an arrowhead.