Fig. 1. Expression of macrophage marker ED2 in testicular co-culture (3D-TCS) over time.
Total protein was collected from testicular co-cultures at various timepoints from initial plating of cells (“Prep day”) to 7 days in culture. Using Western blots to probe for ED1 and ED2 expression, continuous expression of ED2 (but not ED1) was observed. Blot is representative of 3 independent experiments.